Journal: Biomedicines

Article Title: Validation of Serum Biomarkers That Complement CA19-9 in Detecting Early Pancreatic Cancer Using Electrochemiluminescent-Based Multiplex Immunoassays

doi: 10.3390/biomedicines9121897

Figure Lengend Snippet: Analysis of biomarkers in sera from PDAC patients, benign conditions, and healthy controls. ( A – I ), CA-125, HE4, KRT19, FOLR1, CEA, HGF, OPG, Tie-2, and CA19-9 in PDAC patients, benign conditions, and healthy controls are demonstrated in overlaid scatterplots and boxplots. Only serum levels of biomarkers demonstrating significant differences between CP, IPMN, and early stage PDAC, (or benign and PDAC) are asterisked (Mann–Whitney U test). Biomarker data were transformed prior to analysis (log-transformation followed by z-score). Bars indicate median value. *, p < 0.05; **, p < 0.01; ****, p < 0.0001.

Article Snippet: The recombinant proteins and antibodies used for the development of the first 4-plex assay were purchased from the following commercial sources: recombinant human CA125/MUC16 (5609-MU-050), recombinant human FOLR1 protein (5646-FR-050), human FOLR1 antibodies (MAB5646 and AF5646), and human cytokeratin 19 antibody (MAB3506) were from R&D Systems (Minneapolis, MN, USA); CK19 recombinant protein (MBS355584) and mouse CA125 monoclonal antibodies (MBS568141 and MBS568096) were from MyBioSource (San Diego, CA, USA); mouse HE4 monoclonal antibodies (3C24 and 2B13) were from Advanced ImmunoChemical (Long Beach, CA, USA); recombinant human HE4 (230-3001-10) from RayBiotech (Norcross, GA, USA); and mouse Cyfra-21-1 antibody (ABIN573309) from Antibody-Online (Atlanta, GA, USA).

Techniques: MANN-WHITNEY, Biomarker Assay, Transformation Assay

Journal: Biomedicines

Article Title: Validation of Serum Biomarkers That Complement CA19-9 in Detecting Early Pancreatic Cancer Using Electrochemiluminescent-Based Multiplex Immunoassays

doi: 10.3390/biomedicines9121897

Figure Lengend Snippet: Diagnostic performance of individual or combination of serum biomarkers in detection of early stage PDAC. Diagnostic performance of CA19-9, CA-125, HE4, KRT19, FOLR1, CEA, HGF, OPG, and Tie-2 as individual markers ( A , C , E , G , I ) and their complementary ( B , D , F , H , J ) in differentiating patients with benign versus healthy controls (A&B) or PDAC versus benign ( C , D ) or IPMN versus CP ( E , F ) or PDAC versus IPMN ( G , H ) or early stage PDAC versus IPMN ( I , J ). ROC curves with AUCs are presented along with their 95% CI in brackets. Logistic regression modeling was constructed including sex and age as cofactors and backward stepwise selected z-score transformed variables with the highest performance. The four-marker panel of HE4, Tie-2, OPG and HGF including age as a cofactor with an AUC = 0.881 (0.831–0.931) for benign versus healthy controls, the three-marker panel of CA19-9, CA-125 and HE4 including age as a cofactor with an AUC = 0.854 (0.806–0.901) for PDAC versus benign, the two-marker panel of HE4 and CEA including sex and age as cofactors with an AUC = 0.841 (0.767–0.915) for IPMN versus CP, the two-marker panel of CA19-9 and CA-125 with an AUC = 0.857 (0.803–0.911) for PDAC versus IPMN, and the two-marker panel of CA19-9 and CA-125 with an AUC = 0.805 (0.720–0.891) for early stage PDAC versus IPMN significantly improved the individual biomarker performance ( p value: <0.0001, 0.0004, <0.0001, 0.0005, or 0.0113 for CA19-9; Delong test).

Article Snippet: The recombinant proteins and antibodies used for the development of the first 4-plex assay were purchased from the following commercial sources: recombinant human CA125/MUC16 (5609-MU-050), recombinant human FOLR1 protein (5646-FR-050), human FOLR1 antibodies (MAB5646 and AF5646), and human cytokeratin 19 antibody (MAB3506) were from R&D Systems (Minneapolis, MN, USA); CK19 recombinant protein (MBS355584) and mouse CA125 monoclonal antibodies (MBS568141 and MBS568096) were from MyBioSource (San Diego, CA, USA); mouse HE4 monoclonal antibodies (3C24 and 2B13) were from Advanced ImmunoChemical (Long Beach, CA, USA); recombinant human HE4 (230-3001-10) from RayBiotech (Norcross, GA, USA); and mouse Cyfra-21-1 antibody (ABIN573309) from Antibody-Online (Atlanta, GA, USA).

Techniques: Diagnostic Assay, Construct, Transformation Assay, Marker, Biomarker Assay